8種細(xì)菌性肺炎多重檢測試劑盒（PCR方法）

廣州健侖生物科技有限公司

Two tube multiplex for detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella pneumophila/ Legionella longbeachae and internal control.
雙管多重檢測肺炎鏈球菌，流感嗜血桿菌，粘膜炎莫拉氏菌，金黃色葡萄球菌，肺炎支原體，肺炎衣原體，嗜肺軍團(tuán)桿菌，長灘軍團(tuán)菌和內(nèi)部對照。

8種細(xì)菌性肺炎多重檢測試劑盒（PCR方法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

3、“陰陽臉”著色
指組織一半著色一半無著色，形成交界清晰或不甚清晰的兩種染色結(jié)果。其成因是試劑僅覆蓋了部分組織而不是全部。如加試劑后未讓試劑流散開而集中在部分組織上。通常應(yīng)該在加完試劑后，仔細(xì)看一遍，是否有的組織未被試劑*覆蓋，如有這種情況，建議用牙簽而不是用吸頭或試劑瓶口將試劑引流開使之將組織全部覆蓋。另外，染片盒不平，切片傾斜，雖然開始試劑已全部覆蓋了組織，但后來試劑流向一邊，部分組織未被試劑覆蓋。對于這種問題，只要留心或想到了很容易發(fā)現(xiàn)，也很容易解決。有時，用DAKO（或PAP）筆在組織周圍畫圈時，劃線太靠近或畫到了組織上，由于筆油的力學(xué)原理，試劑不能達(dá)到靠近劃線附近的組織。還有氣泡也可引起陰陽分明的著色，只是不著色區(qū)域是圓形，由于氣泡中含氣，試劑被推到周圍，因此，氣泡中心的組織不著色。解決辦法是滴加試劑時手法要輕，有氣泡時用牙簽捅破。
4、灶片狀著色
切片中著色區(qū)東一塊西一塊，呈灶片狀分布，出現(xiàn)這種問題的原因有：（1）裱片時水未排盡，在局部形成氣泡使組織突起，染色時試劑滲入后不易洗盡，顯色過深所致。解決辦法是，漂片盒里的氣泡應(yīng)去盡，晾片熱臺不能平放，應(yīng)有45度左右的斜度，利于水流走和蒸發(fā)。（2）壞死組織灶，組織壞死后細(xì)胞破壞、酶的釋放、蛋白游離、分解，復(fù)雜的肽鏈殘段（如Fc段）可能與一抗或/和二抗結(jié)合導(dǎo)致zui終著色。解決辦法是在選擇染色切片時應(yīng)避免選擇壞死組織較多的切片。（3）制作APES膠片時，膠的濃度太高，干燥后在玻片上留下白色小點(diǎn)，顯色時白色小點(diǎn)著色。解決辦法是按照標(biāo)準(zhǔn)的制備方法進(jìn)行，即5%鹽酸酒精（5 ml鹽酸+95%酒精95 ml）浸泡玻片4小時、熱水沖洗玻片1小時、蒸餾水洗玻片1分鐘、丙酮浸泡玻片5秒鐘后空氣干燥（室溫）、2% APES（2 ml APES+98 ml丙酮）浸泡玻片5分鐘、玻片過一下丙酮（1-2秒鐘）、玻片過一下蒸餾水（1-2秒鐘）、37 度過夜干燥、室溫儲存?zhèn)溆?。如果制片過程中，因丙酮逐漸揮發(fā)而膠變濃時可適當(dāng)加入一些丙酮。

3, "Yin and Yang Face" coloring
Refers to the organization half of the color half without coloring, forming a clear or unclear junction of the two staining results. The reason is that the reagent covers only part of the tissue but not all. Such as adding reagents did not let the reagent flow and focus on part of the organization. Usually after adding the reagent, carefully read it again. Is there any tissue that is not fully covered by the reagent? In such cases, it is advisable to use a toothpick instead of using a tip or a reagent bottle to drain the reagent so that the entire tissue is covered . In addition, the cartridges were uneven and the slides were sloped. Although initially the reagent had compley covered the tissue, the reagents then flowed to one side, and some of the tissue was uncoated by the reagents. For such problems, as long as the care or thought of it is easy to find, but also very easy to solve. Sometimes, with a DAKO (or PAP) pen around the tissue, the scribe line is too close to or drawn onto the tissue, and because of the mechanics of the pen oil, the reagent can not reach the tissue near the scribe line. There are also bubbles can also cause a clear shade of yin and yang, but the non-colored area is round, because the bubbles in the gas, the reagent is pushed around, therefore, the center of the bubble tissue is not colored. The solution is to drop the reagent when the method is lighter, with a toothpick when pierced with bubbles.
4, stovepipe coloring
Eastern section of the coloring area of ??a piece, was distributed in the form of a heart-shaped plate, the reasons for such problems are as follows: (1) when the water is not drained sheeting, the formation of local bubbles in the tissue protrusion, staining difficult to wash after reagent infiltration , Color due to too deep. The solution is, the bubble in the box should go to do, drying tablets can not be put flat heat, should be about 45 degrees of inclination, which will help the water flow and evaporation. (2) necrotic tissue, cell damage after tissue necrosis, enzyme release, protein free, decomposition, complex peptide chain fragments (such as Fc fragment) may be combined with the primary antibody or / and secondary antibody resulting in the final color. The solution is to avoid the need to select more sections of necrotic tissue when choosing a stained section. (3) When making APES film, the concentration of glue is too high, leaving small white spots on the glass slides after drying, and coloring of the white dots when coloring. The solution is according to the standard preparation method, namely 5% hydrochloric acid alcohol (5 ml hydrochloric acid + 95% alcohol 95 ml) for 4 hours soaking the glass slides, hot water slurries for 1 hour, distilled water, slides for 1 minute, acetone immersion glass After 5 seconds, the sample is air-dried (room temperature), 2% APES (2 ml APES + 98 ml acetone) for 5 minutes, slides in the glass for 1-2 seconds, slides in distilled water (1- 2 seconds), 37 degrees overnight dry, room temperature reserve. If the production process, due to the gradual evaporation of acetone and glue thickening may be appropriate to add some acetone.